Understanding Polymer-Cell Attachment

Active screen plasma nitriding enhances cell attachment to polymer surfaces

Active Screen Plasma Nitriding (ASPN) is a well-established technique used for the surface modification of materials, the result of which is often a product with enhanced functional performance. Here we report the modification of the chemical and mechanical properties of ultra-high molecular weight poly(ethylene) (UHMWPE) using 80:20 (v/v) N2/H2 ASPN, followed by growth of 3T3 fibroblasts on th...

Understanding Polymer Processing

Attachment and emotional understanding in preschool children.

This study was designed to elucidate the association between attachment and emotional understanding in preschool children. Forty children between the ages of 2.5 and 6 years and their mothers participated in the study. Mothers completed the Attachment Q-set, and children took part at their preschools in both an affective perspective-taking task and a series of interviews concerning naturally oc...

Endothelial cell attachment and shear response on biomimetic polymer-coated vascular grafts.

Endothelial cell (EC) adhesion, shear retention, morphology, and hemostatic gene expression on fibronectin (FN) and RGD fluorosurfactant polymer (FSP)-coated expanded polytetrafluoroethylene grafts were investigated using an in vitro perfusion system. ECs were sodded on both types of grafts and exposed to 8 dyn/cm(2) of shear stress. After 24 h, the EC retention on RGD-FSP-coated grafts was 59 ...

Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer

BACKGROUND Several cell lines and primary cultures benefit from the use of positively charged extracellular matrix proteins or polymers that enhance their ability to attach to culture plates. Polyethyleneimine is a positively charged polymer that has gained recent attention as a transfection reagent. A less known use of this cationic polymer as an attachment factor was explored with several cel...